Fewer Bacteria Adhere to Softer Hydrogels
Staphylococcus aureus
Microscopy, Confocal
Elastic Modulus
Escherichia coli
Methacrylates
Hydrogels
02 engineering and technology
0210 nano-technology
Bacterial Adhesion
Polyethylene Glycols
3. Good health
DOI:
10.1021/acsami.5b04269
Publication Date:
2015-08-20T15:36:39Z
AUTHORS (3)
ABSTRACT
Clinically, biofilm-associated infections commonly form on intravascular catheters and other hydrogel surfaces. The overuse of antibiotics to treat these has led the spread antibiotic resistance underscores importance developing alternative strategies that delay onset biofilm formation. Previously, it been reported during surface contact, bacteria can detect surfaces through subtle changes in function their motors. However, how stiffness a polymer influences initial attachment is unknown. Systematically, we investigated poly(ethylene glycol) dimethacrylate (PEGDMA) agar hydrogels were 20 times thicker than cumulative size bacterial cell appendages, as Young's moduli. Soft (44.05–308.5 kPa), intermediate (1495–2877 stiff (5152–6489 kPa) synthesized. Escherichia coli Staphylococcus aureus onto was analyzed using confocal microscopy after 2 24 h incubation periods. Independent chemistry time, E. S. correlated positively increasing stiffness. For example, period, there 52 82% fewer adhered soft PEGDMA hydrogels, respectively. A 62 79% reduction area coverage by Gram-positive microbe occurred versus hydrogels. We suggest an easily tunable variable could potentially be used synergistically with traditional antimicrobial reduce early adhesion therefore occurrence infections.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (57)
CITATIONS (116)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....