Electrophoretic Deposition of Dexamethasone-Loaded Mesoporous Silica Nanoparticles onto Poly(l-Lactic Acid)/Poly(ε-Caprolactone) Composite Scaffold for Bone Tissue Engineering

• DOI: 10.1021/acsami.5b11879
• Publication Date: 2016-01-06T19:58:58Z
• Authors: Kexin Qiu; Bo Chen; Wei Nie; Xiaojun Zhou; Wei Feng; Weizhong Wang; Liang Chen; Xiumei Mo; Youzhen Wei; Chuanglong He
• Keywords: Microcarrier; Biocompatibility; Caprolactone; Bioactive Glass

The incorporation of microcarriers as drug delivery vehicles into polymeric scaffold for bone regeneration has aroused increasing interest. In this study, the aminated mesoporous silica nanoparticles (MSNs-NH2) were prepared and used dexamethasone (DEX) loading. Poly(l-lactic acid)/poly(ε-caprolactone) (PLLA/PCL) nanofibrous was fabricated via thermally induced phase separation (TIPS) served template, onto which drug-loaded MSNs-NH2 deposited by electrophoretic deposition (EPD). physicochemical release properties scaffolds (DEX@MSNs-NH2/PLLA/PCL) examined, their osteogenic activities also evaluated through in vitro vivo studies. DEX from revealed an initial rapid followed a slower sustained one. results indicated that DEX@MSNs-NH2/PLLA/PCL exhibited good biocompatibility to rat marrow-derived mesenchymal stem cells (BMSCs). Also, BMSCs cultured on higher degree differentiation than those PLLA/PCL MSNs-NH2/PLLA/PCL scaffolds, terms alkaline phosphatase (ALP) activity, mineralized matrix formation, osteocalcin (OCN) expression. Furthermore, calvarial defect model Sprague–Dawley (SD) rats demonstrated could significantly promote healing compared with scaffold. Thus, EPD technique provides convenient way incorporate agents-containing polymer scaffold, thus, composite be potential candidate tissue engineering application due its capacity agents.