Sensitive Insulin Detection based on Electrogenerated Chemiluminescence Resonance Energy Transfer between Ru(bpy)32+ and Au Nanoparticle-Doped β-Cyclodextrin-Pb (II) Metal–Organic Framework

• DOI: 10.1021/acsami.5b11991
• Publication Date: 2016-04-11T14:11:00Z
• Authors: Hongmin Ma; Xiaojian Li; Tao Yan; Yan Li; Haiyang Liu; Yong Zhang; Dan Wu; Bin Du; Qin Wei
• Keywords: Luminescence; beta-Cyclodextrins; Metal Nanoparticles; 01 natural sciences; 7. Clean energy; 0104 chemical sciences; Energy Transfer; Lead; Luminescent Measurements; Organometallic Compounds; Insulin; Ruthenium Compounds

A novel sandwich-type electrochemiluminescent (ECL) biosensor based on ECL resonance energy transfer (RET) was developed for the determination of insulin. Au nanoparticle-doped Pb(II)-β-cyclodextrin (Pb-β-CD) metal–organic framework was exploited as an ECL donor and chitosan/Ru(bpy)32+/silica nanoparticles (CRuSi NPs) were employed as ECL acceptor. Pb-β-CD shows excellent ECL behavior and unexpected reducing ability toward AuCl4–. Au nanoparticles could massively form on the surface of Pb-β-CD (Au@Pb-β-CD) without use of any additional reducing agent. In the presence of co-reactant K2S2O8, the ECL emission of Pb-β-CD was enhanced by the formation of Au nanoparticles, which is desirable for the construction of a RET immunosensor. The absorption spectra of the synthesized CRuSi NPs show great overlaps with the ECL spectra of Pb-β-CD. This enabled the ECL-RET by incorporating Au@Pb-β-CD and CRuSi NPs into electrode surfaces. The immunosensor is fabricated using Au@Pb-β-CD as sensing platform and CRuSi NPs as...