Effects of Chemical Pretreatments of Wood Cellulose Nanofibrils on Protein Adsorption and Biological Outcomes

• DOI: 10.1021/acsami.5c00391
• Publication Date: 2025-01-30T19:38:36Z
• Authors: Ahmad Rashad; Miina Ojansivu; Ebrahim Afyounian; Ellinor Bævre Heg...; Kristin Syverud; Kamal Mustafa
• Keywords: Nanocellulose; Protein Adsorption; Chemical modification

Wood-based nanocellulose is emerging as a promising nanomaterial in the field of tissue engineering due to its unique properties and versatile applications. Previously, we used TEMPO-mediated oxidation (TO) carboxymethylation (CM) chemical pretreatments prior mechanical fibrillation wood-based cellulose nanofibrils (CNFs) produce scaffolds with different surface chemistries. The aim current study was evaluate effects these on serum protein adsorption 2D 3D configurations TO-CNF CM-CNF then investigate their cell adhesion, spreading, inflammatory mediator production vitro, development foreign body reaction (FBR) vivo. Mass spectrometry analysis revealed that chemistry played key role determining proteomic profile significantly influenced behavior periodontal ligament fibroblasts osteoblast-like cells (Saos-2). samples showed highest followed by samples. adsorbed higher number proteins than counterparts. None CNF toxicity vitro or However, pretreatment negatively affected morphology, spreading both types. Although materials displayed clear differences profiles, triggered same response after being subcutaneously implanted rats for 90 days. This observation highlights degradation rate plays central maintaining It imperative comprehend impact CNFs interaction diverse host types investigation potential modifications. knowledge indispensable advancement regenerative applications within engineering.