Platelet-Rich Plasma-Derived Exosome-Encapsulated Hydrogels Accelerate Diabetic Wound Healing by Inhibiting Fibroblast Ferroptosis

• DOI: 10.1021/acsami.5c02705
• Publication Date: 2025-05-02T16:31:42Z
• Authors: Shanzheng Wang; Jianyue Wu; Ke Ren; Yuehou Zhang; Feifei Gao; Yaofei Chen; Changhong Chen; Jun Lu

Platelet-rich plasma-derived exosomes (PRP-Exos) have recently been considered an optimized strategy for diabetic wound treatment, yet the potential role of PRP-Exos in healing is still unclear. This study aims to investigate mechanisms and utilize hydrogel Pluronic F127 as a carrier maintain sustained release encapsulated PRP-Exos. were isolated from blood healthy individuals characterized, followed by co-culturing with human skin fibroblasts (Diabetes HSF). RNA sequencing (RNA-seq) was used analyze effect on transcriptome normal Diabetes HSF, screening validating crucial mechanism target gene. Then, composed (PRP-Exos/Gel) constructed applied mouse models evaluate mechanism. RNA-seq analysis revealed that significantly upregulated expression FosB HSF. Further intervention HSF showed knocking down induced ferroptosis characterized decreased cell viability, increased oxidative stress, iron ion levels, along downregulation GPX4 SLC7A11 expression, while ACSL4 increased; conversely, overexpression had opposite effect. Subsequently, adding FosB-knocked weakened inhibitory fibroblasts. The synthesized PRP-Exos/Gel exhibited significant thermosensitivity exosomes. In animal experiments, anti-inflammatory effects, evidenced proportion M2 macrophages central granule cells tissue, inhibited fibroblast ferroptosis, thereby accelerating healing. Overall, displays continuous promotes suppressing inflammatory responses which provides new insights methods treatment wounds.