Polymer-Based Raman/PET Dual-Modal Probe for Preoperative Tumor Diagnosis and Intraoperative Image-Guided Surgery and Phototherapy

• DOI: 10.1021/acsami.5c03752
• Publication Date: 2025-05-13T18:47:20Z
• Authors: Yuanyuan Qiu; Qi Zhu; Kai Cui; Haoze Wang; Wenxian Zhang; Xinyi Li; Jiapei Yu; Yumeng Li; Yating Luo; Yiyuan Wang; Wenhui Xie; Qian Xia; Zeyu Xiao

Integration of Raman imaging and positron emission tomography (PET) holds great promise for providing complementary information precise cancer diagnosis imaging-guided therapy. However, current existing Raman/PET dual-modal probes primarily rely on surface-enhanced scattering, raising clinical concerns about the biosafety substrates. Herein, we developed a novel substrate-free probe-[18F]-AS-DPPT-TT NPs, which integrates biocompatible polymer DPPT-TT that possesses strong signals photothermal effects, along with radiolabeling by short-lived 18F radionuclide functionalization tumor-targeting AS1411 aptamer. The [18F]-AS-DPPT-TT NPs generate ultrasensitive resonance under 785 nm excitation, due to their absorption peak closely matching excitation light, exhibit significant effects tumor cell ablation 808 excitation. In orthotopic colon mouse models, enabled preoperative PET high-contrast whole-body localization simultaneously provided intraoperative accurate boundary delineation micrometastasis detection (as small as 0.58 mm × 0.32 mm). Moreover, surgery combined therapy achieved complete elimination primary metastatic tumors, significantly decreasing recurrence rates. This probe effectively combines strengths imaging, robust platform comprehensive management from planning intervention.