Defect-Related Luminescent Hydroxyapatite-Enhanced Osteogenic Differentiation of Bone Mesenchymal Stem Cells Via an ATP-Induced cAMP/PKA Pathway

• DOI: 10.1021/acsami.6b01103
• Publication Date: 2016-04-18T16:44:07Z
• Authors: Chao Wang; Dandan Liu; Cuimiao Zhang; Jiadong Sun; Weipei Feng; Xing-Jie Liang; Shuxiang Wang; Jinchao Zhang
• Keywords: RUNX2; DHAP; Adenosine triphosphate

Novel defect-related hydroxyapatite (DHAP), which combines the advantages of HAP and luminescence, has potential application in tissue engineering biomedical area, because its excellent capability monitoring osteogenic differentiation material biodegradation. Although extracellular mechanism DHAP minerals PO43– functioning been widely studied, intracellular molecular through mediates osteogenesis bone mesenchymal stem cells (BMSCs) is not clear. We examined a previously unknown promoted BMSCs with an emphasis on adenosine–triphosphate (ATP)-induced cAMP/PKA pathway. Our studies showed that could be uptaken into lysosome, was released from DHAP, acid environment lysosome. The interacted ADP to form ATP, then degraded adenosine, ATP metabolite, A2b adenosine receptor activate pathway, resulting high expression osteogenesis-related genes, such as Runx2, BMP-2, OCN. These findings first revealed function ATP-metabolism physiological homeostasis, may developed cure metabolic diseases.