Reversible and Dynamic Fluorescence Imaging of Cellular Redox Self-Regulation Using Fast-Responsive Near-Infrared Ge-Pyronines

• DOI: 10.1021/acsami.6b01348
• Publication Date: 2016-03-21T12:21:44Z
• Authors: Hailiang Nie; Jing Jing; Yong Tian; Wen Yang; Rubo Zhang; Xiaoling Zhang
• Keywords: Fluorescence-lifetime imaging microscopy; Quantum yield

Cellular self-regulation of reactive oxygen species (ROS) stress via glutathione (GSH) antioxidant repair plays a crucial role in maintaining redox balance, which affects various physiological and pathological pathways. In this work, we developed simple yet effective strategy for reversible, dynamic, real-time fluorescence imaging ROS GSH repair, based on novel Ge-pyronine dyes (GePs). Unlike the current O-pyronine (OP) dye, GePs can be quenched reduction then greatly restored by (e.g., ClO–, ONOO–, HO•) oxidation because their unique affinity toward thiols. The "on–off" "off–on" switch complete 10 20 s, respectively, exhibit excellent reversibility vitro cells. also show excitation long wavelength from deep-red to near-infrared (NIR) (621–662 nm) region, high quantum yield (Φfl = 0.32–0.44) aqueous media, cell permeability. Our results demonstrated that used monitoring reversible dynamic interconversion between living might useful tool investigating redox-related