Microfluidic Immuno-Biochip for Detection of Breast Cancer Biomarkers Using Hierarchical Composite of Porous Graphene and Titanium Dioxide Nanofibers

• DOI: 10.1021/acsami.6b05648
• Publication Date: 2016-07-21T16:21:28Z
• Authors: Md. Azahar Ali; Kunal Mondal; Yueyi Jiao; Seval Oren; Zhen Xu; Ashutosh Sharma; Liang Dong
• Keywords: Biochip; Titanium Dioxide

We report on a label-free microfluidic immunosensor with femtomolar sensitivity and high selectivity for early detection of epidermal growth factor receptor 2 (EGFR2 or ErbB2) proteins. This sensor utilizes uniquely structured immunoelectrode made porous hierarchical graphene foam (GF) modified electrospun carbon-doped titanium dioxide nanofibers (nTiO2) as an electrochemical working electrode. Due to excellent biocompatibility, intrinsic surface defects, reaction kinetics, good stability proteins, anatase nTiO2 are ideal applications. The three-dimensional features GF allow penetrate attach the by physical adsorption. Combining functional yields charge transfer resistance, large area, access sensing analyte, resulting in new possibilities development immunosensors. Here, enabling EDC-NHS chemistry covalently immobilized antibody ErbB2 (anti-ErbB2) GF-nTiO2 composite. To obtain compact architecture, composite electrode was designed hang above gold counter channel. underwent differential pulse voltammetry impedance spectroscopy quantify breast cancer biomarkers. two methods had sensitivities 0.585 μA μM(-1) cm(-2) 43.7 kΩ wide concentration range target antigen from 1 × 10(-15) M (1.0 fM) 0.1 10(-6) (0.1 μM) 10(-13) pM) μM), respectively. Utilization specific recognition element, i.e., anti-ErbB2, results specificity, even presence identical members EGFR family tyrosine kinases, such ErbB3 ErbB4. Many promising applications field chemical biological species will derive integration into devices.