Imaging and Intracellular Tracking of Cancer-Derived Exosomes Using Single-Molecule Localization-Based Super-Resolution Microscope

• DOI: 10.1021/acsami.6b09442
• Publication Date: 2016-09-12T15:16:23Z
• Authors: Chen Chen; Shenfei Zong; Zhuyuan Wang; Ju Lu; Dan Zhu; Yizhi Zhang; Yiping Cui
• Keywords: Exosome; Colocalization; Photoactivated localization microscopy

Exosomes are small membrane vesicles secreted by cells and enriched with plenty of proteins. Considering their significant roles in different physical activities potential value for diagnostic drug delivery, researchers have put great efforts vitro tracking content analysis exosomes. Recently, the emergence kinds super-resolution microscopy provides powerful tools exosome study. Here, we demonstrate application single-molecule localization based imaging technique cancer-derived In experiment, first, exosomes extracted from culture media tumor cells. Then receptors labeled photoswitchable probes, which allow these via photoactivated (PALM) or stochastic optical reconstruction (STORM). By using human breast cancer cell-derived exosomes, demonstrated simultaneous dual-color PALM/STORM two on membrane. Moreover, successful labeling make it possible to observe interaction between normal Meanwhile, realized colocalization lysosomes recipient imaging. Since play a vital role intercellular communications, anticipate that presented PALM/STORM-based holds investigation mechanism exosome-mediated metastasis.