Additively Manufactured Macroporous Titanium with Silver-Releasing Micro-/Nanoporous Surface for Multipurpose Infection Control and Bone Repair – A Proof of Concept

• DOI: 10.1021/acsami.6b10473
• Publication Date: 2016-10-05T18:43:07Z
• Authors: Zhaojun Jia; Peng Xiu; Pan Xiong; Wenhao Zhou; Yan Cheng; Shicheng Wei; Yufeng Zheng; Tingfei Xi; Hong Cai; Zhongjun Liu; Caimei Wang; Weiping Zhang; Zhijiang Li
• Keywords: Nanoporous; Proof of concept; Surface Modification

Restoring large-scale bone defects, where osteogenesis is slow while infections lurk, with biomaterials represents a formidable challenge in orthopedic clinics. Here, we propose scaffold-based multipurpose anti-infection and repairing strategy to meet such restorative needs. To do this, personalized multifunctional titanium meshes were produced through an advanced additive manufacturing process dual "TiO2–poly(dopamine)/Ag (nano)" post modifications, yielding macroporous constructs micro-/nanoporous walls nanosilver bullets immobilized/embedded therein. Ultrahigh loading capacity durable release of Ag+ accomplished. The scaffolds active against planktonic/adherent bacteria (Gram-negative positive) for up 12 weeks. Additionally, they not only defended themselves from biofilm colonization but also helped destroy existing biofilms, especially combination antibiotics. Further, the osteoblasts/bacteria coculture study displayed that engineered surfaces aided MG-63 cells combat bacterial invasion. Meanwhile, elicited generally acceptable biocompatibility (cell adhesion, proliferation, viability) hastened osteoblast differentiation maturation (alkaline phosphatase production, matrix secretion, calcification), by synergy micro-/nanoscale topological cues bioactive catecholamine chemistry. Although done ex vivo, these studies reveal our three-in-one (infection prophylaxis, infection fighting, repair) has great potential simultaneously prevent/combat bridge defected bone. This work provides new thoughts use enabling technologies design resolve unmet clinical