Gelatin-Derived Graphene–Silicate Hybrid Materials Are Biocompatible and Synergistically Promote BMP9-Induced Osteogenic Differentiation of Mesenchymal Stem Cells

• DOI: 10.1021/acsami.7b00272
• Publication Date: 2017-04-13T10:17:26Z
• Authors: Yulong Zou; Nader Taheri Qazvini; Kylie Zane; Monirosadat Sadati; Qiang Wei; Junyi Liao; Jiaming Fan; Dongzhe Song; Jianxiang Liu; Chao Ma; Xiangyang Qu; Liqun Chen; Xinyi Yu; Zhicai Zhang; Chen Zhao; Zongyue Zeng; Ruyi Zhang; Shujuan Yan; Tingting Wu; Xingye Wu; Yi Shu; Yasha Li; Wenwen Zhang; Russell R. Reid; Michael J. Lee; Jennifer Moritis ...; Matthew Tirrell; Tong-Chuan He; Juan J. de Pablo; Zhong-Liang Deng
• Keywords: Osteopontin; RUNX2

Graphene-based materials are used in many fields but have found only limited applications biomedicine, including bone tissue engineering. Here, we demonstrate that novel hybrid consisting of gelatin-derived graphene and silicate nanosheets Laponite (GL) biocompatible promote osteogenic differentiation mesenchymal stem cells (MSCs). Homogeneous cell attachment, long-term proliferation, MSCs on a GL-scaffold were confirmed using optical microscopy scanning electron microscopy. GL-powders made by pulverizing the shown to morphogenetic protein (BMP9)-induced differentiation. increased alkaline phosphatase (ALP) activity immortalized mouse embryonic fibroblasts decreased ALP more-differentiated adipose-derived cells. Note, however, promoted BMP9-induced calcium mineral deposits both MSC lines, as assessed qualitative quantitative alizarin red assays. Furthermore, expression chondro-osteogenic regulator markers such Runx2, Sox9, osteopontin, osteocalcin was upregulated GL-powder, independent BMP9 stimulation; although powder synergistically Osterix expression, adipogenic marker PPARγ unaffected. vivo implantation experiments demonstrated GL-powder could significantly enhance ectopic formation from MSCs. Collectively, our results strongly suggest GL hold promise for development engineering platforms.