Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement

• DOI: 10.1021/acsami.7b01555
• Publication Date: 2017-05-12T13:37:58Z
• Authors: Ha-Rim Seo; Hyung Joon Joo; Dae Hwan Kim; Long-Hui Cui; Seung-Cheol Choi; Jong-Ho Kim; Sung Woo Cho; Kyu Back Lee; Do-Sun Lim
• Keywords: Vinculin; Nanopillar; Cofilin

Nanoscaled surface patterning is an emerging potential method of directing the fate stem cells. We adopted nanoscaled pillar gradient patterned cell culture plates with three diameter gradients [280-360 (GP 280/360), 200-280 200/280), and 120-200 nm 120/200)] investigated their fate-modifying effect on multipotent fetal liver kinase 1-positive mesodermal precursor cells (Flk1+ MPCs) derived from embryonic observed increased proliferation colony formation Flk1+ MPCs nanopattern plates. Interestingly, nm-sized 200/280) dramatically cardiomyocyte differentiation expression early cardiac marker gene Mesp1. The surface-induced cardiomyocytes had sarcomeres mature expression. Vinculin p-Cofilin-mediated cytoskeleton reorganization during this process. In summary, pillars enhanced in MPCs.