Immobilization on Metal–Organic Framework Engenders High Sensitivity for Enzymatic Electrochemical Detection

• DOI: 10.1021/acsami.7b02803
• Publication Date: 2017-04-11T16:05:40Z
• Authors: Cheng Zhang; Xuerui Wang; Miao Hou; Xiaoyang Li; Xiaoling Wu; Jun Ge
• Keywords: Cytochromes c; Biosensing Techniques; Electrochemical Techniques; Hydrogen Peroxide; Enzymes, Immobilized; 01 natural sciences; Metal-Organic Frameworks; 0104 chemical sciences

The protection effect of metal–organic framework (MOF) provides high stability for immobilized enzyme. small cavities MOFs, however, usually result in decreased apparent substrate affinity and enzymatic activity enzyme, compared to native We synthesized zeolitic imidazolate framework-8 (ZIF-8) with a combination mesoporous microporous channels cytochrome c (Cyt c) immobilization. Compared Cyt c, the displayed increased (Michaelis constant Km reduced by ∼50%), ∼128% activity, 1.4-fold sensitivity electrochemical detection H2O2. c-coated screen-printed electrode was applied fast residual H2O2 microliter food samples such as milk beer, making it promising development efficient biosensors.