Size-Dependent Translocation of Nanoemulsions via Oral Delivery

• DOI: 10.1021/acsami.7b04916
• Publication Date: 2017-06-15T11:55:57Z
• Authors: Fei Xia; Wufa Fan; Sifan Jiang; Yuhua Ma; Yi Lu; Jianping Qi; Ejaj Ahmad; Xiaochun Dong; Weili Zhao; Wei Wu
• Keywords: Digestion; Microfold cell

The in vivo translocation of nanoemulsions (NEs) was tracked by imaging tools with an emphasis on the size effect. To guarantee accurate identification NEs vivo, water-quenching environment-responsive near-infrared fluorescent probes were used to label NEs. Imaging evidence confirmed prominent digestion gastrointestinal tract and oral absorption integral that survive enteric epithelia a size-dependent way. In general, reducing particle leads slowed vitro lipolysis digestion, prolonged lifetime small intestine, increased epithelial uptake, enhanced transportation various organs. Histological examination revealed pervasive distribution smaller (80 nm) into enterocytes basolateral tissues, whereas bigger ones (550, 1000 primarily adhered villi surfaces. Following are transported through lymphatics fraction approximately 3-6%, suggesting considerable contribution lymphatic pathway overall absorption. majority absorbed found 1 h post administration livers lungs. A similar dependency cellular uptake transmonolayer transport Caco-2 cell lines as well. conclusion, absolute bioavailability at least 6%, envisioning potential applications delivery labile entities.