Free-Energy-Driven Lock/Open Assembly-Based Optical DNA Sensor for Cancer-Related microRNA Detection with a Shortened Time-to-Result

• DOI: 10.1021/acsami.7b06579
• Publication Date: 2017-07-14T13:15:07Z
• Authors: Xiyu Zhu; Ruoyu Wang; Xiaohong Zhou; Hanchang Shi
• Keywords: Lock (firearm)

Quantification of cancer biomarker microRNAs (miRs) by exquisitely designed biosensors with a short time-to-result is great clinical significance. With immobilized capture probes (CPs) and fluorescent-labeled signal (SPs), surface-involved sandwich-type (SST) serve as powerful tools for rapid, highly sensitive, selective detection miR in complex matrices opposed to the conventional techniques. One key challenge such SST existence false-negative signals when amount miRs exceeds SPs solution phase surface limited number CP. To meet this challenge, dynamic lock/open DNA assembly was rationally program pathway miR/SP hybrids. Based on secondary structure analysis free-energy assessment, "locker" strand that partially hybridizes target two separated arms stabilize miR, preventing possible signals. The strategy demonstrated fiber-based fluorescent DNA-sensing platform. CP/miR/SP sandwiches formed fiber would generate quantitative analysis. developed biosensor able detect Hsa let-7a limit 24 pM. applicability free-energy-driven assembly-based optical sensor further confirmed spiked human urine serum samples.