Screening Criteria for Qualified Antibiotic Targets in Unmodified Gold Nanoparticles-Based Aptasensing

• DOI: 10.1021/acsami.7b12796
• Publication Date: 2017-09-28T12:55:05Z
• Authors: Ruoyu Wang; Xiaohong Zhou; Bo Liedberg; Xiyu Zhu; Abdul Ghaffar Memon; Hanchang Shi
• Keywords: 01 natural sciences; 0104 chemical sciences

In designing unmodified gold nanoparticles-based aptasensing (uGA) assays for antibiotics, we find that some antibiotics can adsorb directly on nanoparticles (GNP) regardless of the presence aptamers, which have been long overlooked in past. Some adsorptions, however, would strongly disturb charge distribution GNP surface, break up static colloidal profile, and thus generate false positive colorimetric signals. To identify qualified uGA assays, established two rational screening criteria antibiotic targets relying their oil–water partition coefficients (log P values) net physiological charges: log > 0 ≤0. A good agreement color change was obtained between criteria-based predictions actual tests using six representative antibiotics. The proposed help to shed light GNP–target interactions, is significant developing novel GNP-based with high reliability.