Highly Specific Enrichment of Multi-phosphopeptides by the Diphosphorylated Fructose-Modified Dual-Metal-Centered Zirconium–Organic Framework

• DOI: 10.1021/acsami.8b11138
• Publication Date: 2018-08-30T04:16:15Z
• Authors: Jiaxi Peng; Huan Niu; Hongyan Zhang; Yating Yao; Xingyun Zhao; Xiaoyu Zhou; Lihong Wan; Xiaohui Kang; Ren’an Wu
• Keywords: 01 natural sciences; 0104 chemical sciences

Multisite phosphorylation of a protein, generally occurring in biological processes, plays important roles the regulation cellular functions. However, identification multi-phosphopeptides especially at low abundance is big challenge as extreme hydrophilicity and poor ionization efficiency multiphosphorylated peptides restrict deep inspection multisite processes. In this study, highly specific enrichment was achieved via modification dual-metal-centered zirconium-organic framework with diphosphorylated fructose. The fructose-modified (DZMOF-FDP) demonstrated affinity to multiple phosphorylated peptides, density functional theory calculations explaining plausible mechanism for on DZMOF-FDP. selective capture from mimic samples confirmed superior performance DZMOF-FDP, comprehensive comparisons other agents, such orthophosphate pyrophosphate. A number 1871 captured by DZMOF-FDP tryptic digests HeLa cell lysate could be identified, significantly higher than that pristine DZMOF. deliberately designed fructose dual-zirconium-centered metal-organic materials suggests an efficient strategy develop new methods target analytes judiciously optimizing modifiers adsorbents.