Construction of Injectable Self-Healing Macroporous Hydrogels via a Template-Free Method for Tissue Engineering and Drug Delivery

• DOI: 10.1021/acsami.8b13077
• Publication Date: 2018-09-27T21:25:15Z
• Authors: Lei Wang; Fen Deng; Wenwen Wang; Afeng Li; Conglie Lu; Hao Chen; Gang Wu; Kaihui Nan; Lingli Li
• Keywords: 570; Alginates; Cell Survival; Biocompatible Materials; 02 engineering and technology; Cell Line; Rats, Sprague-Dawley; Mice; Drug Delivery Systems; Glucuronic Acid; Animals; Humans; Schiff Bases; Epidermal Growth Factor; Temperature; Hydrogels; 540; Rats; 3. Good health; Oxygen; NIH 3T3 Cells; Gelatin; SDG 6 - Clean Water and Sanitation; Rheology; 0210 nano-technology; Porosity

Because of their ease handling and excellent biocompatibility, injectable macroporous hydrogels have received a considerable interest in the fields tissue engineering drug delivery systems because unique application minimally invasive surgical procedures. In this study, situ forming, injectable, macroporous, self-healing gelatin (GE)/oxidized alginate (OSA)/adipic acid dihydrazide (ADH) were prepared using high-speed shearing treatment stabilized by Schiff base reaction acylhydrazone bonds. Their injectability, ability, rheology, microstructure, equilibrium water content, vitro biodegradation investigated. We found that GE/OSA/ADH precursors remained liquid form flowed easily for several minutes at room temperature, but however, gelled rapidly body temperature. The gelation time could be regulated varying ratio GE, OSA, ADH. obtained had an interconnected structure ability. porosity was range approximately 60–83%, pore size varied from 125–380 μm. porous hydrogel visualized field-emission scanning electron microscope, micro-computed tomography, laser confocal microscope. Human epidermal growth factor loaded mixing released with good bioactivity as evaluated ELISA. Moreover, L929 cells proliferated on hydrogels, verified Cell Counting Kit-8 LIVE/DEAD assays. Furthermore, encapsulation NIH 3T3 within demonstrated can support cell survival, proliferation, migration. vivo studies showed gelation, biocompatibility. Therefore, represented novel safe scaffold vehicle purposes.