DNA-Functionalized Porous Fe3O4 Nanoparticles for the Construction of Self-Powered miRNA Biosensor with Target Recycling Amplification

• DOI: 10.1021/acsami.8b15419
• Publication Date: 2018-10-10T17:56:16Z
• Authors: Tian Zhang; Hua Chai; Fanyu Meng; Zhenzhen Guo; Yu Jiang; Peng Miao
• Keywords: Metal Nanoparticles; Biosensing Techniques; DNA; Electrochemical Techniques; Ferric Compounds; 01 natural sciences; Catalysis; 0104 chemical sciences; 12. Responsible consumption; Glucose Oxidase; MicroRNAs; Limit of Detection; Humans; Graphite; Gold; Electrodes; Oxidation-Reduction; Porosity

Herein, we have developed an ultrasensitive self-powered biosensor for miRNA assay based on biofuel cells. The system is composed of indium tin oxide cathode and graphene oxide/gold nanoparticle/glucose oxidase anode. Redox probe [Fe(CN)6]3- entrapped inside porous Fe3O4 nanoparticles by DNA. However, in the presence target miRNA, hybridization reaction occurs between DNA, which initiates release [Fe(CN)6]3-. Moreover, duplex specific nuclease further employed to trigger recycling amplification. As a result, much more redox probes are released open circuit voltage significantly increased. A "signal-on" quantification thus developed. detection range from 10 aM fM; meanwhile, limit as low 1.4 aM, superior that most reported methods. Therefore, proposed expected be powerful point-of-care tool diagnostics, may wide applications future.