Controlled Nanoscale Topographies for Osteogenic Differentiation of Mesenchymal Stem Cells

• DOI: 10.1021/acsami.8b21393
• Publication Date: 2019-02-20T22:19:09Z
• Authors: Catarina R. Pedrosa; Didier Arl; Patrick Grysan; Irfan Khan; Stéphanie Durrieu; Sivashankar Krish...; Marie-Christine D...
• Keywords: Adult; 0301 basic medicine; Silicon; Pyridines; topographies; osteogenic differentiation; Bone Marrow Cells; Core Binding Factor Alpha 1 Subunit; [SDV.BC]Life Sciences [q-bio]/Cellular Biology; Collagen Type I; 03 medical and health sciences; nanoscale topography; Osteogenesis; Humans; block copolymer self-assembly; [SDV.BC] Life Sciences [q-bio]/Cellular Biology; Cells, Cultured; Aged; mesenchymal stem cells; [CHIM.MATE] Chemical Sciences/Material chemistry; Cell Differentiation; Mesenchymal Stem Cells; [CHIM.MATE]Chemical Sciences/Material chemistry; nanoscale; Nanostructures; Collagen Type I, alpha 1 Chain; Microscopy, Fluorescence; Tissue Array Analysis; Polystyrenes; Osteopontin; Polyvinyls

Nanotopography with length scales of the order extracellular matrix elements offers possibility regulating cell behavior. Investigation impact nanotopography on response has been limited by inability to precisely control geometries, especially at high spatial resolutions and across practically large areas. In this paper, we demonstrate well-controlled periodic nanopillar arrays silicon investigate their osteogenic differentiation human mesenchymal stem cells (hMSCs). Silicon critical dimensions in range 40–200 nm, exhibiting standard deviations below 15% full wafers, were realized using self-assembly block copolymer colloids. Immunofluorescence quantitative polymerase chain reaction measurements reveal clear dependence hMSCs diameter periodicity arrays. Further, was found be dependent age donor. While osteoblastic promoted pillars larger diameters heights independent donor age, they different for spacings. Pillar smaller pitch from a young donor, while spacing those an old These findings can contribute development personalized treatments bone diseases, namely, novel implant nanostructuring depending patient age.