Novel Mussel-Inspired Universal Surface Functionalization Strategy: Protein-Based Coating with Residue-Specific Post-Translational Modification in Vivo

• DOI: 10.1021/acsami.8b22551
• Publication Date: 2019-03-07T11:19:03Z
• Authors: Haishan Qi; Weiwei Zheng; Chen Zhang; Xiao Zhou; Lei Zhang
• Keywords: Surface Modification; Biocompatibility; Protein Adsorption; Biofouling; Bioconjugation

Surface functionalization can effectively endow materials with desirable properties, promoting the performance between material and environment, extensive applications. However, a universal straightforward surface method biocompatibility is scarce. In this study, synthetic biology strategy, recombinant mussel plaque protein zwitterionic peptide inspired by molecular chaperone was engineered through post-translational modification, in which 3,4-dihydroxyphenylalanine residue-specifically obtained efficiently from tyrosine tyrosinase coexpressed vivo. The rational designed chimeric coating work could successfully anchor to various substrates exhibit excellent antifouling resisting adsorption, cell attachment, bacterial adhesion eminent biocompatibility.