Three-Dimensional Printed Scaffolds with Controlled Micro-/Nanoporous Surface Topography Direct Chondrogenic and Osteogenic Differentiation of Mesenchymal Stem Cells

• DOI: 10.1021/acsami.9b01472
• Publication Date: 2019-05-08T17:14:01Z
• Authors: Aruna Prasopthum; Mick Cooper; Kevin M. Shakesheff; Jing Yang
• Keywords: Chondrogenesis; Nanoporous; 3d printed

The effect of topography in three-dimensional (3D) printed polymer scaffolds on stem cell differentiation is a significantly underexplored area. Compared to two-dimensional (2D) biomaterials which various well-defined topographies have been incorporated and shown direct range behaviors including adhesion, cytoskeleton organization, differentiation, incorporating topographical features 3D challenging due the difficulty accessing inside porous scaffold. Only roughened strut surface has introduced scaffolds. Here, rapid, single-step printing method fabricate polymeric consisting microstruts (ca. 60 μm) with micro-/nanosurface pores (0.2–2.4 developed based ink writing an agitated viscous solution. density, size, alignment these can be controlled by changing degree agitation or speed printing. Three-dimensional micro-/nanoporous struts enhanced chondrogenic osteogenic mesenchymal cells (MSCs) without soluble factors. also selectively affected morphology, MSC lineages depending composition medium. This fabrication potentially used for wide polymers where desirable architecture are required.