Recombinant Human Hair Keratin Nanoparticles Accelerate Dermal Wound Healing
Male
0301 basic medicine
Wound Healing
Keratins, Type II
Dermis
02 engineering and technology
Recombinant Proteins
Cell Line
Rats
3. Good health
Rats, Sprague-Dawley
03 medical and health sciences
Keratins, Hair-Specific
Keratins, Type I
Animals
Humans
Nanoparticles
Wounds and Injuries
0210 nano-technology
Cell Proliferation
DOI:
10.1021/acsami.9b01725
Publication Date:
2019-04-30T19:27:24Z
AUTHORS (7)
ABSTRACT
In recent years, favorable enhanced wound-healing properties and excellent biocompatibility of keratin derived from human hair have attracted considerable attention. Recombinant keratin proteins can be produced by recombinant DNA technology and have higher purity than extracted keratin. However, the wound-healing properties of recombinant keratin proteins remain unclear. Herein, two recombinant trichocyte keratins including human type I hair keratin 37 and human type II hair keratin 81 were expressed using a bacterial expression system, and recombinant keratin nanoparticles (RKNPs) were prepared via an ultrasonic dispersion method. The molecular weight, purity, and physicochemical properties of the recombinant keratin proteins and nanoparticles were assessed using gel electrophoresis, circular dichroism, mass spectrometry, and scanning electron microscope analyses. The RKNPs significantly enhanced cell proliferation and migration in vitro, and the treatment of dermal wounds in vivo with RKNPs resulted in improved wound healing associated with improved epithelialization, vascularization, and collagen deposition and remodeling. In addition, the in vivo biocompatibility test revealed no systemic toxicity. Overall, this work demonstrates that RKNPs are a promising candidate for enhanced wound healing, and this study opens up new prospects for the development of keratin biomaterials.
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