Recombinant Human Hair Keratin Nanoparticles Accelerate Dermal Wound Healing

• DOI: 10.1021/acsami.9b01725
• Publication Date: 2019-04-30T19:27:24Z
• Authors: Feiyan Gao; Wenfeng Li; Jia Deng; Jinlan Kan; Tingwang Guo; Bochu Wang; Shilei Hao
• Keywords: Biocompatibility; Keratin 6A

In recent years, favorable enhanced wound-healing properties and excellent biocompatibility of keratin derived from human hair have attracted considerable attention. Recombinant proteins can be produced by recombinant DNA technology higher purity than extracted keratin. However, the remain unclear. Herein, two trichocyte keratins including type I 37 II 81 were expressed using a bacterial expression system, nanoparticles (RKNPs) prepared via an ultrasonic dispersion method. The molecular weight, purity, physicochemical assessed gel electrophoresis, circular dichroism, mass spectrometry, scanning electron microscope analyses. RKNPs significantly cell proliferation migration in vitro, treatment dermal wounds vivo with resulted improved wound healing associated epithelialization, vascularization, collagen deposition remodeling. addition, test revealed no systemic toxicity. Overall, this work demonstrates that are promising candidate for healing, study opens up new prospects development biomaterials.