Recombinant Human Hair Keratin Nanoparticles Accelerate Dermal Wound Healing

• DOI: 10.1021/acsami.9b01725
• Publication Date: 2019-04-30T19:27:24Z
• Authors: Feiyan Gao; Wenfeng Li; Jia Deng; Jinlan Kan; Tingwang Guo; Bochu Wang; Shilei Hao
• Keywords: Male; 0301 basic medicine; Wound Healing; Keratins, Type II; Dermis; 02 engineering and technology; Recombinant Proteins; Cell Line; Rats; 3. Good health; Rats, Sprague-Dawley; 03 medical and health sciences; Keratins, Hair-Specific; Keratins, Type I; Animals; Humans; Nanoparticles; Wounds and Injuries; 0210 nano-technology; Cell Proliferation

In recent years, favorable enhanced wound-healing properties and excellent biocompatibility of keratin derived from human hair have attracted considerable attention. Recombinant keratin proteins can be produced by recombinant DNA technology and have higher purity than extracted keratin. However, the wound-healing properties of recombinant keratin proteins remain unclear. Herein, two recombinant trichocyte keratins including human type I hair keratin 37 and human type II hair keratin 81 were expressed using a bacterial expression system, and recombinant keratin nanoparticles (RKNPs) were prepared via an ultrasonic dispersion method. The molecular weight, purity, and physicochemical properties of the recombinant keratin proteins and nanoparticles were assessed using gel electrophoresis, circular dichroism, mass spectrometry, and scanning electron microscope analyses. The RKNPs significantly enhanced cell proliferation and migration in vitro, and the treatment of dermal wounds in vivo with RKNPs resulted in improved wound healing associated with improved epithelialization, vascularization, and collagen deposition and remodeling. In addition, the in vivo biocompatibility test revealed no systemic toxicity. Overall, this work demonstrates that RKNPs are a promising candidate for enhanced wound healing, and this study opens up new prospects for the development of keratin biomaterials.