DNA-Based Nanomedicine with Targeting and Enhancement of Therapeutic Efficacy of Breast Cancer Cells

• DOI: 10.1021/acsami.9b03449
• Publication Date: 2019-03-29T10:02:25Z
• Authors: Yuxi Zhan; Wenjuan Ma; Yuxin Zhang; Chenchen Mao; Xiaoru Shao; Xueping Xie; Fei Wang; Xiaoguo Liu; Qian Li; Yunfeng Lin
• Keywords: Aptamer

Recently, a DNA tetrahedron has been reported to be novel nanomedicine and promising drug vector because of its compactness, biocompatibility, biosafety, editability. Here, we modified the with aptamer (AS1411) as DNA-based delivery system, which could bind nucleolin for cancer cell selectivity. Nucleolin is specific biomarker protein overexpressed on membranes malignant cells deregulation implicated in proliferation. The antimetabolite 5-fluorouracil (5-FU) an extensively used anticancer agent; however, major limitation lack target specificity. Cyanine 5 (Cy5), fluorescent probe, can label enhance photostability minimal effects basic functions. In this study, additionally attached 5-FU system new tumor-targeting (AS1411-T-5-FU) therapeutic efficacy targeting breast cancer. We examined difference cellular uptake AS1411-T-5-FU between normal concluded that had better ability kill than 5-FU. further evaluated expressions apoptosis-related proteins genes, are associated mitochondrial apoptotic pathway. Ultimately, our results suggest potential therapies, develop approach endow property.