Alginate Lyase Guided Silver Nanocomposites for Eradicating Pseudomonas aeruginosa from Lungs

• DOI: 10.1021/acsami.9b21815
• Publication Date: 2020-02-06T09:47:31Z
• Authors: Bing Wan; Ying Zhu; Jun Tao; Feipeng Zhu; Jianquan Chen; Li Li; Jianfeng Zhao; Li Wang; Shuangshuang Sun; Yang Yang; Xiuwei Zhang; Yunlei Zhang
• Keywords: Biocompatibility

Pseudomonas aeruginosa (P. aeruginosa) infections lead to a high mortality rate for cystic fibrosis or immunocompromised patients. The alginate of the biofilm was believed be key factor disabling immune therapy and antibiotic treatments. A silver nanocomposite consisting nanoparticles mesoporous organosilica layer created deliver two pharmaceutical compounds (alginate lyase ceftazidime) degrade eradicate P. from lungs. introduction thioether-bridged into nanocomposites greatly benefited conjunction foreign functional molecules such as increased their hemocompatibility drug-loading capacity. Silver with uniform diameter (∼39 nm) exhibited dispersity, good biocompatibility, ceftazidime-loading capacity (380.96 mg/g). Notably, displayed low pH-dependent drug release degradation profiles (pH 6.4), guaranteeing targeted drugs in acidic niches biofilm. Indeed, particles loaded ceftazidime inhibitory effects on PAO1 based specific catalytic activity enzyme antibacterial function ions. It should noted that enzyme-decorated succeeded eradicating mouse lungs decreasing lung injuries. No deaths serious side were observed during experiments. We believe biocompatibility organic group-incorporated framework have potential used multiple clinical application.