Influence of Charge and Heat on the Mechanical Properties of Scaffolds from Ionic Complexation of Chitosan and Carboxymethyl Cellulose

• DOI: 10.1021/acsbiomaterials.1c00534
• Publication Date: 2021-07-15T18:34:18Z
• Authors: Andreja Dobaj Šti...; Rupert Kargl; Marco Beaumont; Christine Strauss; Damjan Makuc; Dominik Egger; Janez Plavec; Orlando J. Rojas; Karin Stana Klein...; Tamilselvan Mohan
• Keywords: Carboxymethyl cellulose

As one of the most abundant, multifunctional biological polymers, polysaccharides are considered promising materials to prepare tissue engineering scaffolds. When properly designed, wetted porous scaffolds can have biomechanics similar living and provide suitable fluid transport, both which key features for in vitro vivo growth. They further mimic components function glycosaminoglycans found extracellular matrix tissues. In this study, we investigate formed by charge complexation between anionic carboxymethyl cellulose cationic protonated chitosan under well-controlled conditions. Freeze-drying dehydrothermal heat treatment were then used obtain with exceptional, unprecendent mechanical properties dimensional long-term stability cell growth media. We investigated how conditions, ratio, significantly influence resulting uptake biomechanics. Surprisingly, high compressive strength, elastic modulus, significant shape recovery obtained certain address mostly a balanced ratio formation covalent amide bonds polymers without use additional cross-linkers. The promoted clustered adhesion showed no cytotoxic effects as assessed viability assay live/dead staining human adipose tissue-derived mesenchymal stem cells. suggest that or biomaterials comprising other large potential cartilage elucidating reason observed peculiar stimulate research.