Nuclear Deformability of Breast Cells Analyzed from Patients with Malignant and Benign Breast Diseases

• DOI: 10.1021/acsbiomaterials.2c01059
• Publication Date: 2023-01-27T17:58:37Z
• Authors: Ezgi Antmen; Menekse Ermis; Ozgur Kuren; Kemal Beksac; Cigdem Irkkan; Vasif Hasirci
• Keywords: MUC1; Breast cancer metastasis

Breast cancer is a heterogeneous and dynamic disease, in which cells are highly responsive to alterations the microenvironment. Today, conventional methods of detecting give rather static image condition so properties such as invasiveness metastasis difficult capture. In this study, molecular-level evaluations patients with breast adenocarcinoma were combined vitro on micropatterned poly(methyl methacrylate) (PMMA) biomaterial surfaces that deform cells. A correlation between deformability nuclei stemness, invasiveness, was sought. Clinical patient samples from regions different proximities tumor. Responses at single-cell level toward studied using CD44/24, epithelial cell adhesion marker (EpCAM), MUC1, PCK. Results showed molecular markers shape descriptors can discriminate tumor center patients. The most metastatic invasive both highest markers. conclusion, by combination together nuclear deformation, it possible improve detection separation subpopulations heterogenous specimens level.