Deep Learning-Predicted Dihydroartemisinin Rescues Osteoporosis by Maintaining Mesenchymal Stem Cell Stemness through Activating Histone 3 Lys 9 Acetylation

• DOI: 10.1021/acscentsci.3c00794
• Publication Date: 2023-10-18T12:01:53Z
• Authors: Ruoxi Wang; Yu Wang; Yuting Niu; Danqing He; Shanshan Jin; Zixin Li; Lisha Zhu; Liyuan Chen; Xiaolan Wu; Chengye Ding; Tianhao Wu; Xinmeng Shi; He Zhang; Chang Li; Xin Wang; Zhengwei Xie; Weiran Li; Yan Liu
• Keywords: Chemistry; QD1-999; 3. Good health

Maintaining the stemness of bone marrow mesenchymal stem cells (BMMSCs) is crucial for homeostasis and regeneration. However, in vitro expansion diseases impair BMMSC stemness, limiting its functionality tissue engineering. Using a deep learning-based efficacy prediction system sequencing, we identify natural small-molecule compound, dihydroartemisinin (DHA), that maintains enhances During long-term expansion, DHA preserves characteristics, including self-renewal ability unbiased differentiation. In an osteoporosis mouse model, oral administration restores femur trabecular structure, density, situ. Mechanistically, by promoting histone 3 lysine 9 acetylation via GCN5 activation both vivo vitro. Furthermore, bone-targeted delivery mesoporous silica nanoparticles improves therapeutic osteoporosis. Collectively, could be promising agent treating maintaining stemness.