Rescuing the Rescuer: On the Protein Complex between the Human Mitochondrial Acyl Carrier Protein and ISD11

• DOI: 10.1021/acschembio.8b00184
• Publication Date: 2018-05-08T14:52:37Z
• Authors: María Georgina He...; María Florencia P...; Martín Ezequiel N...; Karen Magalí Cruz; Javier Santos
• Keywords: Protein Stability; Folding (DSP implementation)

Iron–sulfur clusters are essential cofactors in many biochemical processes. ISD11, one of the subunits protein complex that carries out cluster assembly mitochondria, is necessary for cysteine desulfurase NFS1 stability and function. Several authors have recently provided evidence showing ISD11 interacts with acyl carrier (ACP). We carried coexpression human mitochondrial ACP E. coli. This work shows form a soluble, structured, stable able to bind subunit modulating its activity. Results suggest plays key-role folding vitro. These findings offer opportunity study mechanism interaction between NFS1.