Protein Drug Delivery Using a Novel Maxillofacial Technique Targeting the Visual Pathway in the Brain, the Optic Nerve, and the Retina

• DOI: 10.1021/acschemneuro.3c00184
• Publication Date: 2023-09-04T16:48:31Z
• Authors: Rahini Rajendran; Jayamuruga Pandia...; Subbulakshmi Chid...; Srikanth Krishnag...; Bhavani Krishnamu...; Subha Vinayagam; Veeran Veeravarmal; Harikrishnan Prasad; Kavita Verma; Anoop U.R
• Keywords: Systemic administration

Protein drugs are used for treating many diseases of the eye and brain. The formidable blood neural barriers prevent delivery these into Hence, there is a need protein drug system to deliver large proteins across blood-neural barriers. Low half-life, poor penetration epithelial barriers, low stability, immunogenicity limit use non-invasive systemic routes delivering proteins. In this pre-clinical study, efficacy new maxillofacial route administering using novel compared with administration through intra-peritoneal injection ocular topical drops subconjunctival intravitreal injections. Bevacizumab retinoschisin were administered technique along in wild-type male C57BL/6J mice. Liquid chromatography tandem mass spectrometry western blot was detect bevacizumab tissue samples. Furthermore, immunohistochemistry performed presence localization retina could target brain including regions involved visual pathway optic nerve. effective retina. A concept based on glymphatic pathway, cerebrospinal fluid distribution, crossover ipsilateral nerve fibers at chiasma proposed explain contralateral following injection.