Characterization of Serine Hydrolases Across Clinical Isolates of Commensal Skin Bacteria Staphylococcus epidermidis Using Activity-Based Protein Profiling

• DOI: 10.1021/acsinfecdis.0c00095
• Publication Date: 2020-04-16T21:34:40Z
• Authors: Laura J. Keller; Christian S. Lentz; Y. Erin Chen; Rebecca J. Metivier; Eranthie Weerapana; Michael A. Fischbach; Matthew Bogyo
• Keywords: Commensalism; Virulence factor

The bacterial genus Staphylococcus comprises diverse species that colonize the skin as commensals but can also cause infection. Previous work identified a family of serine hydrolases termed fluorophoshonate-binding (Fphs) in pathogenic bacteria aureus, one which, FphB, functions virulence factor. Using combination bioinformatics and activity-based protein profiling (ABPP), we identify homologues these enzymes related commensal epidermidis. Two S. aureus Fph were not ABPP, several candidate previously may be functionally to Fphs. Interestingly, activity Fphs vary across clinical isolates Biochemical characterization FphB homologue epidermidis (SeFphB) suggests it is functional our preliminary studies suggest have role colonization vivo. This potential difference biological function between closely staphylococcal provide mechanisms for specific inhibition infection without perturbing communities bacteria.