Nipah Virus V Protein Binding Alters MDA5 Helicase Folding Dynamics

• DOI: 10.1021/acsinfecdis.1c00403
• Publication Date: 2021-12-08T13:00:43Z
• Authors: Nicole D. Wagner; Hejun Liu; Henry W. Rohrs; Gaya K. Amarasinghe; Michael L. Gross; Daisy W. Leung
• Keywords: MDA5; Hendra Virus

Nipah virus (NiV) is an emerging and deadly zoonotic paramyxovirus that responsible for periodic epidemics of acute respiratory illness encephalitis in humans. Previous studies have shown the NiV V protein antagonizes host antiviral immunity, but molecular mechanism incompletely understood. To address this gap, we biochemically characterized binding to pattern recognition receptor MDA5. We find C-terminal domain (V