Synergistic Effect of Formate and Cell-Free Supernatant Fermented by Two Probiotics, Bifidobacterium animalis spp. lactis HN019 and Lacticaseibacillus rhamnosus HN001, against Shigella flexneri Growth

• DOI: 10.1021/acsinfecdis.4c00796
• Publication Date: 2025-05-15T08:44:43Z
• Authors: Ke-Chuan Wang; Christian Clement...; Heli Anglenius; Krista Salli; Wesley Morovic; Matthew Poore; Svetlana Gerdes; Sebastian Meier; Henrik Max Jensen; Pernille Rose Jensen
• Keywords: Bifidobacterium animalis; Lactobacillus rhamnosus; Sapovirus

The interplay between probiotic metabolites, host health, and inhibition of pathogens has increasingly attracted interest but remains unresolved due to the complex molecular interactions among these factors. We investigated action cell-free supernatants (CFSs) from two bacteria, Bifidobacterium animalis spp. lactis (B. lactis) HN019 Lacticaseibacillus rhamnosus (L. rhamnosus) HN001 their effect against bacterial pathogen Shigella flexneri 2457T (S. flexneri). CFSs B. exhibited a higher antibacterial S. growth than L. HN001, independent carbon source utilized. This correlated with formate within HN019. As expected, antimicrobial effects were stronger free cells for infecting cells. A synergistic additional small organic molecules such as indole-3-lactate was found. Such CFSs, indole-3-lactate, reflected by altered metabolic rates in presence both solution under biofilm-forming conditions. different components acting on gives reasons believe that suitably designed mixtures products small-molecule effectors bear promise successfully combating pathogens.