Surface-Enhanced Raman Scattering Active Gold Nanoparticles with Enzyme-Mimicking Activities for Measuring Glucose and Lactate in Living Tissues

• DOI: 10.1021/acsnano.7b00905
• Publication Date: 2017-05-26T22:11:11Z
• Authors: Yihui Hu; Hanjun Cheng; Xiaozhi Zhao; Jiangjiexing Wu; Faheem Muhammad; Shichao Lin; Jian He; Liqi Zhou; Chengping Zhang; Yu Deng; Peng Wang; Zhengyang Zhou; Shuming Nie; Hui Wei
• Keywords: Glucose oxidase

Gold nanoparticles (AuNPs) with simultaneous plasmonic and biocatalytic properties provide a promising approach to developing versatile bioassays. However, the combination of AuNPs' intrinsic enzyme-mimicking their surface-enhanced Raman scattering (SERS) activities has yet be explored. Here we designed peroxidase-mimicking nanozyme by in situ growing AuNPs into highly porous thermally stable metal–organic framework called MIL-101. The obtained AuNPs@MIL-101 nanozymes acted as peroxidase mimics oxidize Raman-inactive reporter leucomalachite green active malachite (MG) hydrogen peroxide simultaneously SERS substrates enhance signals as-produced MG. We then assembled glucose oxidase (GOx) lactate (LOx) onto form AuNPs@MIL-101@GOx AuNPs@MIL-101@LOx integrative for vitro detection via SERS. Moreover, were further explored monitoring change living brains, which are associated ischemic stroke. used evaluate therapeutic efficacy potential drugs (such astaxanthin alleviating cerebral injuries) rats. They also employed determine metabolism tumors. This study not only demonstrated great promise combining multiple functionalities bioassays but provided an interesting designing biomedical catalytic applications.