Synchrotron Fourier Transform Infrared Microscopy Spectra in Cellular Effects of Janus Kinase Inhibitors on Myelofibrosis Cancer Cells

• DOI: 10.1021/acsomega.2c02404
• Publication Date: 2022-06-23T15:51:16Z
• Authors: Jeeraprapa Siriwa...; Kamonpan Sanachai; Thitinan Aiebchun; Lueacha Tabtimmai; Buabarn Kuaprasert; Kiattawee Choowon...
• Keywords: Infrared microscopy; Janus kinase inhibitor

Janus kinase (JAK) deregulation of the JAK/signal transducers and activators transcription pathway leads to myelofibrosis that can be treated by JAK inhibitors including Ruxolitinib Tofacitinib. Even though both are effective against myelofibrosis, each them has a different mode action in cells. is an inhibitor for selective JAK1/2, Tofacitinib JAK3. This study evaluated chemical fingerprints TF-1 cells after treatments synchrotron Fourier transform infrared microspectroscopy (S-FTIR) spectrum. were applied with fingerprint approach S-FTIR spectroscopy vitro cytotoxicity cell-based assay. Principal component analysis or PCA was utilized classify three cell biochemical alteration absorbances lipid vibration C–H stretching, protein amide I appeared from C═O P═O phosphodiester bond nucleic acids. The results showed inhibition effect on lines two-fold higher than distinguishes untreated drug-treated detecting cellular alteration. loading plots identify proteins acids main components disparate treatments. distinct others acid. second derivative spectra molecular had decreased production accumulation, changes secondary structures proteins, high level RNA overexpression treatment. caused spectroscopic biomarkers modifications conformation, stimulated phosphorylation suggests FTIR potential tool analyze specific patterns drug responses at level.