Hyaluronic Acid Viscosupplement Modulates Inflammatory Mediators in Chondrocyte and Macrophage Coculture via MAPK and NF-κB Signaling Pathways

• DOI: 10.1021/acsomega.4c01911
• Publication Date: 2024-05-01T07:32:27Z
• Authors: Sree Samanvitha K...; Ju Yeon Kang; Hong Yeol Yang; Seok Cheol Lee; Jaishree Sankaran...; Hyung Keun Kim; Jong Keun Seon
• Keywords: Proinflammatory cytokine; MMP3; Aggrecan

Osteoarthritis (OA) is a chronic musculoskeletal disorder characterized by cartilage degeneration and synovial inflammation. Paracrine interactions between chondrocytes macrophages play an essential role in the onset progression of OA. In this study, replicating inflammatory response during OA pathogenesis, were treated with interleukin-1β (IL-1β), lipopolysaccharide interferon-γ. addition, coculture system was developed to simulate biological situation joint. we examined impact hyaluronic acid (HA) viscosupplement, particularly Hyruan Plus, on macrophages. Notably, viscosupplement has demonstrated promising outcomes reducing inflammation; however, underlying mechanism action remains elusive. The attenuated inflammation, showing inhibitory effect nitric oxide production, downregulating proinflammatory cytokines such as matrix metalloproteinases (MMP13 MMP3), upregulating expression levels type II collagen aggrecan chondrocytes. HA also reduced level IL-1β, TNF-α, IL-6 macrophages, exerted overall protective partially suppressing MAPK pathway p65/NF-κB signaling Therefore, shows potential for treating arthritic joints.