Mass Spectrometry Imaging Reveals Region-Specific Lipid Alterations in the Mouse Brain in Response to Efavirenz Treatment

• DOI: 10.1021/acsptsci.4c00228
• Publication Date: 2024-07-09T12:51:21Z
• Authors: Nav Raj Phulara; Apurv Rege; Charles J. Bieberich; Herana Kamal Sene...
• Keywords: Efavirenz; Mass spectrometry imaging

Efavirenz (EFV) is a commonly used drug to treat human immunodeficiency virus infection and known exert adverse effects on the brain. Although it that EFV associated with abnormal plasma lipid levels, changes in spatial localization of individual molecules brain tissue following treatment are yet be explored. In this study, we employed matrix-assisted laser desorption/ionization mass spectrometry imaging approach determine region-specific alterations mouse brains treatment. We detected unique patterns phosphatidylcholine (PC), sphingomyelin (SM), ceramide phosphoinositol (PI-Cer), hexosylceramide (HexCer) Interestingly, PC(32:0), PC(38:5), SM(36:1;O2) showed high abundance hippocampus region, whereas PI-Cer(38:8) exhibited low region EFV-treated brains. Additionally, observed PC(38:6), PC(40:6), PI-Cer(40:3) thalamus Furthermore, SM(40:1;O2), SM(42:2;O2), SM(42:1;O2), SM(43:2;O2), SM(43:1;O2) their accumulation corpus callosum as compared controls. However, HexCer(42:1;O3) depletion response To characterize expression proteins, including metabolizing enzymes, treatment, spectrometry-based proteomics was utilized. From these, levels 12 proteins were found significantly decreased Taken together, these multiomics data provide important insights into metabolism.