Insights into the Formation of DNA–Magnetic Nanoparticle Hybrid Structures: Correlations between Morphological Characterization and Output from Magnetic Biosensor Measurements

• DOI: 10.1021/acssensors.0c01623
• Publication Date: 2020-11-03T17:07:28Z
• Authors: Reinier Oropesa-N...; Teresa Zardán Góm...; Henry Stopfel; Peter Svedlindh; Mattias Strömberg; Klas Gunnarsson
• Keywords: Characterization; Biomagnetism; Rolling circle replication

Understanding the binding mechanism between probe-functionalized magnetic nanoparticles (MNPs) and DNA targets or amplification products thereof is essential in optimization of biosensors for detection DNA. Herein, molecular interaction forming hybrid structures upon hybridization DNA-functionalized nanoparticles, exhibiting Brownian relaxation, rolling circle (DNA-coils) investigated by use atomic force microscopy a liquid environment measuring frequency-dependent response modulation light transmission. This approach reveals qualitative quantitative correlations morphological features with their response. The suppression high-frequency peak appearance new at lower frequencies match formation larger sized assemblies increasing concentration DNA-coils. Furthermore, an increase DNA-coil induces number MNPs per structure. study provides insights into DNA–MNP mechanism, its versatility considerable importance mechanistic characterization other DNA-nanoparticle biosensor systems.