Catalytic Hairpin Self-Assembly-Based SERS Sensor Array for the Simultaneous Measurement of Multiple Cancer-Associated miRNAs

• DOI: 10.1021/acssensors.0c01876
• Publication Date: 2020-12-09T04:08:36Z
• Authors: Yanmei Si; Lan Xu; Ting Deng; Jing Zheng; Jishan Li
• Keywords: Sensor array

The abnormal expression of some miRNAs is often closely related to the development tumors. Available detection methods or biosensors that can simultaneously quantify multiple in a single sample have rarely been reported. Herein, novel catalytic hairpin self-assembly (CHA)-based surface-enhanced Raman scattering (SERS) sensor array was developed measure associated with cancer one sample. four different sensing units constructed by immobilizing hairpin-structured DNA sequence 1 (hp1) onto Au/Ag alloy nanoparticle (AuAgNP)-coated wells. When target miRNA present, SERS tags, which were prepared modifying AuAgNPs reporter molecule 4-mercaptobenzonitrile (MPBN) and 2 (hp2), captured corresponding unit through repeated specific CHA reaction. This generated many "hot spots" because interactions between tags AuAgNP layer-coated surface sensor, ultimately produced strong signal allowed limit 0.15 pM. Using this array, cancer-associated (miR-1246, miR-221, miR-133a, miR-21) successfully determined buffer, serum, cellular RNA extracts.