DNA Nanowire Guided-Catalyzed Hairpin Assembly Nanoprobe for In Situ Profiling of Circulating Extracellular Vesicle-Associated MicroRNAs

• DOI: 10.1021/acssensors.1c02717
• Publication Date: 2022-03-21T20:02:24Z
• Authors: Ye Zhang; Yuan Wu; Shihua Luo; Chao Yang; Guangzhi Zhong; Guoni Huang; Xiaohe Zhang; Bo Li; Chunchen Liu; Ling Li; Xiaohui Yan; Lei Zheng; Bo Situ
• Keywords: Nanoprobe; Extracellular vesicles; Extracellular Vesicles

Extracellular vesicle-associated miRNAs (EV-miRNAs) are emerging as a new type of noninvasive biomarker for disease diagnosis. Their relatively low abundance, however, makes accurate detection challenging. Here, we designed DNA nanowire guided-catalyzed hairpin assembly (NgCHA) nanoprobe profiling EV-miRNAs. NgCHA showed high penetrability to EVs, which allowed rapid delivery the probes into EVs. In presence targeted within fluorescent signal could be generated and amplified by confining catalytic system nanowires, thus greatly enhancing analytical sensitivity. We that EV-miRNAs from various cell lines accurately quantified in situ. By using four-EV-miRNA panel, this platform can identify patients with breast cancer at an early stage 95.2% sensitivity 86.7% specificity. Its applications risk assessment well prediction were also successfully demonstrated. This is sensitive, low-cost, simple compared current methods. It may serve promising tool diagnosis monitoring cancers other diseases through EV-miRNA profiling.