Amplification of the Fluorescence Signal with Clustered Regularly Interspaced Short Palindromic Repeats-Cas12a Based on Au Nanoparticle-DNAzyme Probe and On-Site Detection of Pb2+ Via the Photonic Crystal Chip

• DOI: 10.1021/acssensors.2c00516
• Publication Date: 2022-04-28T16:20:09Z
• Authors: Yu-Yao Li; Hao-Dong Li; Wen-Kai Fang; Da Liu; Meng-Han Liu; Ming-Qiu Zheng; Li-Ling Zhang; He Yu; Hong-Wu Tang
• Keywords: Deoxyribozyme; Biomolecule; Detection theory; SIGNAL (programming language)

Although great headway has been made in DNAzyme-based detection of Pb2+, its adaptability, sensitivity, and accessibility complex media still need to be improved. For this, we introduce new ways surmount these hurdles. First, a spherical nucleic acid (SNA) fluorescence probe (Au nanoparticles-DNAzyme probe) is utilized specifically identify Pb2+ suitability for precise samples due excellent nuclease resistance. Second, the sensitivity greatly enhanced via use clustered regularly interspaced short palindromic repeats-Cas12a with target recognition accuracy amplify fluorescent signal upon trans cleavage SNA (signal probe), limit reaches as low 86 fM. Third, boost on photonic crystal chips bionic periodic arrangement by employing straightforward device (smartphone portable UV lamp) achieve on-site 24 pM. Based abovementioned efforts, modified sensor advantages higher better specificity, accessibility, less sample consumption, so forth. Moreover, it can applied accurately detect biological or environmental samples, which promise widespread applications.