Cationic Covalent Organic Polymer Thin Film for Label-free Electrochemical Bacterial Cell Detection

• DOI: 10.1021/acssensors.2c01292
• Publication Date: 2022-09-02T15:42:39Z
• Authors: Tina Skorjanc; Andraž Mavrič; Mads Nybo Sørensen; Gregor Mali; Changzhu Wu; Matjaz Valant
• Keywords: Cationic polymerization; Electrochemical cell

Numerous species of bacteria pose a serious threat to human health and cause several million deaths annually. It is therefore essential have quick, efficient, easily operable methods bacterial cell detection. Herein, we synthesize novel cationic covalent organic polymer (COP) named CATN through the Menshutkin reaction evaluate its potential as an impedance sensor for Escherichia coli cells. On account positive surface charge (ζ-potential = +21.0 mV) pyridinium moieties, expected interact favorably with that possess negatively charged electrostatic interactions. The interdigitated electrode arrays were coated using simple yet non-traditional method electrophoresis then used in two-electrode electrochemical spectroscopy (EIS) measurements. response showed linear relationship increasing concentration E. coli. system was sensitive concentrations low ∼30 CFU mL-1, which far below considered illnesses. calculated limit detection 2 mL-1. This work rare example COP this type sensing anticipated stimulate further interest synthesis polymers EIS-based sensors.