Orange-Emissive Carbon Dots with High Photostability for Mitochondrial Dynamics Tracking in Living Cells

• DOI: 10.1021/acssensors.2c02451
• Publication Date: 2023-02-16T21:06:51Z
• Authors: Nini Xin; Dong Gao; Borui Su; Ting Zhou; Yuda Zhu; Chengheng Wu; Dan Wei; Jing Sun; Hongsong Fan
• Keywords: HeLa; Quantum yield

Mitochondria play significant roles in maintaining a stable internal environment for cell metabolism. Hence, real-time monitoring of the dynamics mitochondria is essential further understanding mitochondria-related diseases. Fluorescent probes provide powerful tools visualizing dynamic processes. However, most mitochondria-targeted are derived from organic molecules with poor photostability, making long-term challenging. Herein, we design novel probe based on carbon dots high performance tracking. Considering that targeting ability CDs related to surface functional groups, which generally determined by reaction precursors, successfully constructed O-CDs emission at 565 nm through solvothermal treatment m-diethylaminophenol. The bright quantum yield 12.61%, mitochondria-targeting ability, and good stability. possess (12.61%), specific outstanding optical Owing abundant hydroxyl ammonium cations surface, showed obvious accumulation colocalization coefficient up 0.90 remained steady even after fixation. Besides, compatibility photostability under various interruptions or long-time irradiation. Therefore, preferable tracking mitochondrial behavior live cells. We first observed fission fusion behaviors HeLa cells, then, size, morphology, distribution physiological pathological conditions were clearly recorded. More importantly, different interactions between lipid droplets during apoptosis mitophagy This study provides potential tool exploring other organelles, promoting research