Single-Walled Carbon Nanotube Sensor Selection for the Detection of MicroRNA Biomarkers for Acute Myocardial Infarction as a Case Study

• DOI: 10.1021/acssensors.3c00633
• Publication Date: 2023-09-13T04:37:16Z
• Authors: Adi Hendler-Neumark; Verena Wulf; Gili Bisker
• Keywords: MicroRNAs; DNA, Complementary; Nanotubes, Carbon; Myocardial Infarction; Humans; DNA; Biomarkers

MicroRNAs (miRNAs) are single-stranded non-coding short ribonucleic acid sequences that take part in many cellular and biological processes. Recent studies have shown altered expression of miRNAs is involved pathological processes, they can thus be considered biomarkers for the early detection various diseases. Here, we demonstrate a selection elimination process fluorescent single-walled carbon nanotube (SWCNT) sensors miRNA based on RNA-DNA hybridization with complementary DNA recognition unit bound to SWCNT surface. We use known acute myocardial infarction (AMI), commonly as heart attack, case study. selected five possible which selective specific AMI tested DNA-SWCNT sensor candidates target RNA different environments. Out these sensors, three could recognize or sequence buffer, showing fluorescence modulation response sequence. working only one function serum was further testing. The chosen sensor, SWCNT-miDNA208a, showed high specificity selectivity toward sequence, better performance compared buffer environment. pipeline highlights importance testing appropriate environment extended other libraries biomarkers.