Development of a Dual Reporter System to Simultaneously Visualize Ca2+ Signals and AMPK Activity

• DOI: 10.1021/acssensors.4c01058
• Publication Date: 2024-08-22T03:17:17Z
• Authors: Yusuf C. Erdoğan; Johannes Pilic; Benjamin Gottschalk; Esra N. Yiğit; Asal G. Zaki; Gürkan Öztürk; Emrah Eroğlu; Begüm Okutan; Nicole G. Sommer; Annelie M. Weinberg; Rainer Schindl; Wolfgang F. Graier; Roland Malli
• Keywords: Phase Separation-Based Activity Reporter Of Kinase; SPARK; Fluorescence Microscopy; AMPK Reporter; Ca2+ Biosensor; Gcamp; Multiplexing

In this study, we introduce a new separation of phases-based activity reporter kinase (SPARK) for AMP-activated (AMPK), named AMPK-SPARK, which reports the AMPK activation by forming bright fluorescent clusters. Furthermore, dual system, GCaMP-AMPK-SPARK, incorporating single-fluorescent protein (FP)-based Ca