SynNotch-Programmed Macrophages for Cancerous Cell Detection and Sensing

• DOI: 10.1021/acssensors.4c01997
• Publication Date: 2024-11-04T21:09:57Z
• Authors: Tao Wang; Cia-Hin Lau; Naian Wang; Jiaqi Li; Jianchao Wang; Zhihao Huang; Weidong Wu; Xiaoqing Chen; Jiahui Li; Minghai Zou; Wenju Zhang; Yulin Li; Jingrong Li; Wenkai Ma; Yumei Huang; Meijing Xu; Haibao Zhu; Gang Chen

Synthetic Notch (synNotch) receptors have enabled mammalian cells to sense extracellular ligands and respond by activating user-prescribed transcriptional programs. Based on the synNotch system, we describe a cell-based in vivo sensor for cancerous cell detection. We attempted engineer synNotch-programmed macrophages cancer via urinary analysis of human chorionic gonadotropin (HCGB5). Principally, when bind cells, is activated undergoes intramembrane proteolysis release activator into nucleus. The targets activates downstream gene expression, such as (HCGB5) macrophages. When HCGB5 secreted extracellularly urine, it can be detected with commercial colloidal gold test strips. As proof principle, demonstrated feasibility detecting breast engineered artificial EGFP ligands. that expression was only induced expressing present; thereby, directly proportional number cells. Further optimizations system realize ultimate goal establishing sensors paragon diagnostics point-of-care testing home self-test.