Customized AIEgen-Based Molecular Signaling Tags Combined Microfluidic Chip for Point-of-Care Testing Viable E. coli O157:H7

• DOI: 10.1021/acssensors.4c02301
• Publication Date: 2025-02-13T05:59:31Z
• Authors: Niu Feng; Yiqi Li; Yongkun Zhao; Jiacheng Tao; Hong Jiang; Shu Wang; Xiaolin Huang; Jimei Ma; Ben Zhong Tang
• Keywords: Point-of-Care Testing; Microfluidic chip; Point of care

Pathogenic bacterial infections pose a significant threat to human life, health, and socioeconomic development, with those arising from Escherichia coli (E. coli) O157:H7 being particularly concerning. Herein, customized aggregation-induced emission luminogens (AIEgen)-based signaling tags (TPA-galactose) were combined microfluidic chip for the determination of E. O157:H7. TPA-galactose undergoes hydrolysis by β-galactosidase, resulting in formation highly fluorescent TPA–OH AIE characteristics. Phages covalently bound surface magnetic beads specifically capture lyse O157:H7, releasing endogenous fluorescence intensity facilitates The process achieves sensitivity 45 CFU/mL min, requiring no DNA extraction or amplification, utilizing minimal sample volume, enabling accurate one-stop quantification live This strategy enables rapid on-site environmental, food, clinical samples, significantly enhancing public health safety.