Au@Fe3O4 Nanoparticle-Based Colorimetric Aptasensor for Noninvasive Screening of Colorectal Cancer via Detection of Parvimonas micra

• DOI: 10.1021/acssensors.4c02885
• Publication Date: 2025-02-05T06:15:26Z
• Authors: Shanshan Feng; Peiyi Zhang; Hui Chen; Bo Zhou; Ying Qin; Tingting Fan; Qinsheng Sun; Yan Chen; Yuyang Jiang
• Keywords: Colorectal cancer screening

Colorectal cancer (CRC) is a common malignancy requiring early screening to improve patient outcomes. Current methods such as colonoscopy and fecal occult blood tests have several limitations including high cost, poor specificity, invasiveness, inconvenience. Recent research has identified specific bacterial communities associated with CRC, notably Parvimonas micra (P. micra), which serves biomarker for diagnosis owing its accumulation in the malignant tissues feces of CRC patients. Herein, we employed whole-bacterium systematic evolution ligands by exponential enrichment (SELEX) method isolate high-affinity aptamers against P. using 17 selection cycles. These were subsequently bound Au@Fe3O4 nanoparticles, interaction inhibited peroxidase-like activity thereby blocking 3,3',5,5'-tetramethylbenzidine (TMB) chromogenic reaction resulting measurable reduction absorbance. This colorimetric detection strategy demonstrated linear response across range 100-108 CFU/mL limit 11 CFU/mL. Using aptasensor, assessed abundance clinical samples found significantly higher levels patients compared that healthy individuals, was consistent quantitative polymerase chain results. study therefore represents first successful identification an aptamer affinity specificity micra, leading development highly sensitive aptasensor detection. The presented approach significant potential diagnosis.