Femtomolar Detection by Nanocoated Fiber Label-Free Biosensors

• DOI: 10.1021/acssensors.7b00918
• Publication Date: 2018-05-04T14:54:26Z
• Authors: Francesco Chiavaioli; Pablo Zubiate; Ignacio Del Villar; Carlos R. Zamarreño; Ambra Giannetti; Sara Tombelli; Cosimo Trono; Francisco J. Arregui; Ignacio R. Matias; Francesco Baldini
• Keywords: Indium tin oxide

The advent of optical fiber-based biosensors combined with that nanotechnologies has provided an opportunity for developing in situ, portable, lightweight, versatile, and high-performance sensing platforms. We report on the generation lossy mode resonances by deposition nanometer-thick metal oxide films fibers, which makes it possible to measure precisely accurately changes properties fiber-surrounding medium very high sensitivity compared other technology platforms, such as long period gratings or surface plasmon resonances, gold standard label-free real-time biomolecular interaction analysis. This property, application specialty structures D-shaped permits enhancing light–matter interaction. SEM TEM imaging together X-EDS tool have been utilized characterize two used, i.e., indium tin dioxide. Moreover, experimental transmission spectra obtained after nanocoatings numerically corroborated means wave propagation methods. With use a conventional wavelength interrogation system ad hoc developed microfluidics, shift resonance can be reliably recorded response low analyte concentrations. Repeated experiments confirm big leap performance thanks capability detect femtomolar concentrations human serum, improving detection limit 3 orders magnitude when configurations. biosensor regenerated several times injecting sodium dodecyl sulfate, proves sensor reused.